Both sides come into the clash in with great form, with Spurs derby victory over Arsenal at Wembley over the weekend.
Spurs reached the knockout stage by topping Group H, which included Borussia Dortmund and current holders Real Madrid while Juventus finished second behind Barcelona in Group D.
The Old Lady have made the Champions League Final in two of the last three seasons, but both times came up short against Spanish opposition as Barcelona and Real Madrid beat them.
Team Injuries
Juventus, Argentine talisman Paulo Dybala is a doubt for the game while Andrea Barzagli, Benedikt Howedes, Blaise Matuidi and Juan Cuadrado have all been ruled out.
Spurs defender Toby Alderweireld is unavailable but otherwise, Premier League outfit have no major injury worries.
Head to Head
Juventus and Tottenham have both won one and lost one of their two previous meetings, although both were friendly fixtures.
Recent Team Form
Juventus has won all their last six matches, while Spurs win three and draw the same number from their last six encounters.
Manager Quotes Juve boss Massimiliano Allegri said: "We know their strengths and we know that we will have to suffer for some parts of the game because Tottenham have
four players with great quality in attack.
"We have achieved very good results in Europe. Only Real Madrid have done better in my opinion in recent years."
Spurs manager Mauricio Pochettino said: "He had a massive injury and from day one we designed a plan to provide him with the best tools to recover as soon as possible and to be stronger than before,"
"He has started to work with the team and it's our judgement that he needs to build his fitness."
Possible Juventus starting XI: Buffon; Rugani, Chiellini, Benatia, Sandro; Marchisio, Pjanic, Khedira; Bernardeschi, Higuain, Mandzukic.
Possible Tottenham starting XI: Lloris, Davies, Vertonghen, Sanchez, Dier, Aurier, Dembele, Alli, Eriksen, Son, Kane.
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